Question: pls answer all 3 questions Peptide A: HYEFDVIM Peptide B: HYHFKVIM Which of the following types of chromatography would be expected to be useful to

pls answer all 3 questions Peptide A: HYEFDVIM Peptide B: HYHFKVIM Which of the following types of chromatography would be expected to be useful to separate these two peptides from each other at pH 5? (Each pls answer all 3 questions

Peptide A: HYEFDVIM Peptide B: HYHFKVIM Which of the following types of chromatography would be expected to be useful to separate these two peptides from each other at pH 5? (Each correctly assigned choice earns 0.2 marks.) Anion exchange chromatography Cation exchange chromatography Affinity chromatography Size exclusion chromatography Hydrophobic interaction chromatography What is the net charge of this peptide at pH 5? Use the pKa values from Table 4-1 from Voet, Voet and Pratt. 2 1 0 +1 +2 Calculate the isoelectric point of Peptide A using the pKa values in Table 4-1 of Voet, Voet and Pratt. Assume that the pKa values for the N-terminal amino group and C terminal carboxyl groups are 8.0 and 3.5, as previously discussed in class. 10.247.025.0553.7853.50

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