Question: pls explain You will use DLS to characterize the size of your synthesized liposomes. In this lab, you use PC and PE to produce large
You will use DLS to characterize the size of your synthesized liposomes. In this lab, you use PC and PE to produce large multilamellar vesicles (MLVs), freeze/thaw them to produce smaller liposomes, and then extrude them through a 200 nm pore filter to form large unilamellar vesicles (LUVS). You will use DLS before and after extruding to determine the size and polydispersity (breadth of distribution of sizes). The polydispersity is defined in DLS as the ratio of: (standard deviation of the diameter measurements) / (average diameter). Suppose you get two measurements: A: diameter - 350 nm; PDI -0.014 B: diameter - 947 nm; PDI -0.216 What would you conclude from the data? liposomes in sample A are smaller than in B, and mostly all the same size o liposomes in sample B are smaller than in A, and mostly all the same size O liposomes in sample A are smaller than in B, but have wildly varying sizes o liposomes in sample B are smaller than in A, but have wildly varying sizes
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