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Answer ALL questions 1) Production of human insulin by a genetically engineered strain of Escherichia coli (E.coli) is proportional to cell growth. Bacteria consume all of the limiting reactant under aerobic conditions and produce insulin and biomass. Ammonia is used as nitrogen source for aerobic respiration of glucose. 50mM Ammonia is used as nitrogen source for aerobic respiration of 150 mM glucose and feed to the bioreactor at a rate of 100 Lh. The insulin has an overall formula 10, N. The cell biomass molecular formula is given as CH,9093No.3. Pure oxygen enters the reactor as a gas at the same molar flow rate as the ammonia. The respiratory quotient RO = 0.50 mol CO, produced mol O, consumed, and the molar ratio of biomass to insulin produced in the reaction is 1.5. The system operates at steady state. Wnite the stoichiometry equation and determine the stoichiometry coefficients for each components. (10 marks) b. Calculate the fractional conversion of glucose? (10 marks) If the product stream leaves at a rate of 100 Lh and the RQ value is 0.50, what is the production rates (gh) of insulin and biomass? (10 marks) 2) A new environmental engineering company comce to you to get an advice on incinerator system. They would like to use a mixed of bwardous waste and primary fuel as a feed for a boiler in the plant. The hazardous waste containing 70 wt% of sand and 30 wt% of polychlorinated biphenyls (PCB) is to be cleaned by combustion with the excess of hexanc in order to produce a feed that is 60% combustible by weight. An oxygen-enriched air stream comprising 40 mol% of oxygen and 60 mol% of nitrogen is supplied to the incinerator in order produce high temperature operation. The exit gas from the incinerator is found to contain a 16.5 mol% of carbon dioxide and 12.2% of Oxygen. 8. Draw a complete process flow diagram (labelled appropriately) and write chemical reaction equation(s) that would take place in the system. (10 marks) Perform a complete degree of freedom analysis (5 marks) b. 2 KKKR1123/KKPK1133 c. The company would like to know the amount of hexane would be required to decontaminate 5000kg of the contaminated sand. Calculate the amount of hexane required. (5 marks) d. Find the complete exit gas concentrations. (10 marks) e. Calculate the percentage of oxygen excess used in the reactions, (5 marks) 3) You have been employed as a chemical engineer in a chemical plant that producing ethylenc oxide in Pengerang, Johor. Your first job is to design the catalytic oxidation process of ethylene to produce the Ethylene oxide. There are two chemical reactions take place in the reactor as shown below: 2CH. +02 -- 2CHO C2H4 + 30,- 2002 + 2H20 The feed to the reactor (not the fresh feed to the process) contains 3 moles of ethylenc per mole of oxygen. The single-pass conversion of cthylenc is 20%, and for every 100 molcs of cthylene consumed in the reactor, 90 moles of ethylenc oxide emerge in the reactor products. A multiple- unit process is used to separate the products: ethylene and oxygen arc recycled to the reactor, cthylenc oxide is sold as a product and carbon dioxide and water are discarded. 9. Draw a complete process flow diagram (labelled appropriately) and perform a complete degree of freedom analysis (10 marks) b. Determine the molar flow rates of ethylene and oxygen in the fresh feed (10 marks) c. Determine the production rate of ethylenc oxide (Smarks) d. Determine the feed the overall conversion of ethylene. (5 marks) e. Determine the molar flow rates of ethylene and oxygen in the fresh feed needed to produce 1 ton per hour of ethylene oxide