Predict your results. Write "yes" or "no," depending on whether you think the plate will show growth. Give the reason (s) for your predictions. Observe the colonies through the petri plate lids. Do not open the plates. Prediction: Prediction: Reason: LB-plasmid Reason: LB+plasmid 1 2 Observed Result: Observed Result: Prediction: Prediction: LB/amp -plasmid Reason: LB/amp +plasmid Reason: 3. Observed Result: Observed Result: Record your observed results in the spaces above. If your observed results differed from your predictions, explain what you think may have occurred. Count the number of individual colonies and, using a permanent marker, mark each colony as it is counted. If the cell growth is too dense to count individual colonies, record "lawn." LB+plasmid (Positive Control) LB/Amp+plasmid (Experimental) LB-plasmid (Positive Control) LB/Amp-plasmid (Negative Control) Compare and contrast the number of colonies on each of the following pairs of plates. What does each pair of results tell you about the experiment? a. LB+plasmid and LB-plasmid b. LB/Amp-plasmid and LB-plasmid c. LB/Amp+plasmid and LB/Amp-plasmid d. LB/Amp+plasmid and LB+plasmid d. LB/Amp+plasmia and LD+piasmia What are you selecting for in this experiment? (i.e., what allows you to identify which bacteria have taken up the plasmid?) What does the phenotype of the transformed colonies tell you? What one plate would you first inspect to conclude that the transformation occurred successfully? Why?