Problem 1 (CB 154) Growth Phase Deceleration Exponential Death or Decline LO Stationary 11 O Particle Counter . Plate (Viable Cell Counts Optical 1o Density 0.1 8 log Cell Number / mL 1. use Monod Model to describe the lag phase, exponential phase and stationary phase, and write the differential equation or integral equation for each phase. 2. use 1st-order kinetics to describe the death phase, and derive the integral equation from the ODE. 3. estimate the kinetics constant Hm according to the plot of cell concentration (determination of cell number based on Plate Counts) against time. 4. calculate the doubling time of the cell from the above data. Hints for Problem 1 Step 2: develop kinetics equations for each phase Step 2: data collection from the figure given: Step 3: use Excel or origin Dato-processing software to calculate rates or specific rates Step 4: estimate the kinetics constant 3 0.01 10 15 Time (h) vie. Typical growth curve for bacterial in Note that the phone prowth shows here forced her dress on the parte do monitor