Question: Problem 2 ( 3 5 points ) . 2 0 0 liters of a solution containing 1 0 g ? liter of and antibody and

Problem 2(35 points).200 liters of a solution containing 10g? liter of and antibody and 5g? liter of a
second protein are to be treated with ammonium sulfate. The objective is to recover 90% of the
antibody in the precipitate. The following solubility data is available
The solubility of the impurity is not affected by pHH
a) What is the intrinsic solubility of the antibody at pH5 and at pH8?
b) Assuming that coprecipitation is negligible, at what pH would you perform the precipitation and
what will be the purity of the antibody in the precipitate?
Problem 2(35 points).200 liters of a solution containing 10g? liter of

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