Propose a complete analytical protocol (sample preparation, separation and detection) for the determination of clomipramine and its metabolites in plasma samples. In the proposed separation system, it must include a stationary and mobile phase. Motivate your answer. Answer: For sample preparation Solid phase extraction (SPE) can be used. For separation a C18 column kan be used with isocratic flow 50%water and 50% methanol. For detection masspectrometry can be used. It's the "motivate your answer" part that I need help with. Why choose to use SPE, C18 column, isocratic flow and masspectrometry? Please motivate and explain.