Protein Purification Biochemistry Lab

For each section below, make an entry that addresses the points given in each paragraph. Make sure your paragraphs flow!

Introduction

• What was the purpose of this study?
• What is already known about this topic?
• What techniques did you use in this study?
• How did the techniques you used fulfill the purpose of this study??

Tests and Procedures

• What did youdo to achieve the purpose of this study?
• For this section, state what you did in the protein purification lab.The idea is to keep records that are detailed enough so that anyone with a similar background to yours can repeat exactly what you did.

The table below shows a table of absorbances for my Bradford assay.

Table of Absorbances for Bradford Assay:

Sample	Amount (?l)	Absorbance at 595 nm
Protein Standard 0 mg/ml	20	0
Protein Standard 0.125 mg/ml	20	0.567
Protein Standard 0.25 mg/ml	20	0.601
Protein Standard 0.5 mg/ml	20	0.730
Protein Standard 0.75 mg/ml	20	0.854
Protein Standard 1.0 mg/ml	20	0.930
Protein Standard 1.5 mg/ml	20	1.374
Protein Standard 2.0 mg/ml	20
Extract 1/10	20	0.838
Extract 1/100	20	0.604
Extract 1/1000	20	0.577
Blue Fraction (undiluted)	20	0.804
Blue Fraction 1/10	20	0.616
Blue Fraction 1/100	20	0.561
Yellow Fraction (undiluted)	20	0.567
Yellow Fraction 1/10	20	0.558
Yellow Fraction 1/100	20	0.547

The picture below is an image of my gel.

Image of gel

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