Provide a systematic approach for the general operating procedure for liquid chromatography starting with mobile phase preparation till sample injection. Your approach should highlight all the necessary precautions required to ensure both the best data acquisition but also the long-term preventive maintenance of the equipment. 3 marks

You have been tasked to quantify the amount of capsaicin present in a sample of dried chillies. Methanol extracts have been carried out and are ready for analysis and a total of 10 samples need to be analysed. You have been provided with pure capsaicin powder and a certified standard at 1000 ppm stock concentration and from literature it was found that range of capsaicin in chilli vary between 50-500 ppm. Write down a typical sample list which you must input in the software. The list should include the following.

System suitability, precision, calibration curve, samples, intermediate precision, accuracy, and shutdown method.