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Bumble Bee Foods has voluntarily recalled some of his canned tuna fish products because they may have been undercooked due to equipment malfunction in the sterilization process, which could lead to life-threatening illness if consumed. The engineers suggested the recall because the 5 oz. cans were exposed to a temperature of 121.1 degree C for less than the required sterilization time, which they had previously determined experimentally. The heating process was designed based on an initial population of spores of 4x10^6 and actual population vs. time data. Time min 0 2 4 6 8 10 12 14 16 18 N (number of spores) 4000000 400000 80000 16000 3500 1000 200 70 10 3 Construct a microbial inactivation curve (log N vs process time, t) and determine the D value for the process. Calculate the required heating time to achieve commercial sterilization of the canned tuna products. Were the engineers correct? Decimal reduction time, D value, is the time required to reduce the viable population by a factor of 10 (i.e., one log). It is determined from the inverse of the slope of the microbial inactivation plot. log N/No = - t/D The required heating process time for Clostridium Botulinum was established as 12D Bumble Bee Foods has voluntarily recalled some of his canned tuna fish products because they may have been undercooked due to equipment malfunction in the sterilization process, which could lead to life-threatening illness if consumed. The engineers suggested the recall because the 5 oz. cans were exposed to a temperature of 121.1 degree C for less than the required sterilization time, which they had previously determined experimentally. The heating process was designed based on an initial population of spores of 4x10^6 and actual population vs. time data. Time min 0 2 4 6 8 10 12 14 16 18 N (number of spores) 4000000 400000 80000 16000 3500 1000 200 70 10 3 Construct a microbial inactivation curve (log N vs process time, t) and determine the D value for the process. Calculate the required heating time to achieve commercial sterilization of the canned tuna products. Were the engineers correct? Decimal reduction time, D value, is the time required to reduce the viable population by a factor of 10 (i.e., one log). It is determined from the inverse of the slope of the microbial inactivation plot. log N/No = - t/D The required heating process time for Clostridium Botulinum was established as 12D