Question: Q5 lon Chromatography A 20 L sample solution, containing approximately 3 mM sodium nitrate and 2 mM calcium sulfate in water, was injected into

Q5 lon Chromatography A 20 L sample solution, containing approximately 3 mM

Q5 lon Chromatography A 20 L sample solution, containing approximately 3 mM sodium nitrate and 2 mM calcium sulfate in water, was injected into an anion exchange chromatograph and eluted with 8.0 mM KOH at a flow of 1.2 mL/min. The column had a capacity of 30 ueq and void volume of 2.0 mL. The system and resulting chromatograph is shown below. a) Explain why the peaks in the top chromatogram are negative, whereas the peaks in the bottom chromatogram are positive. (2) b) According to theory, which peak should be the broadest. Why? (4) c) How would an AERS suppressor work in this system? (3) d) Calculate the effective ion exchange site concentration of the column, Ca, in meq/L. (1) Calculate the width of the sample plug at injection, in seconds. (2) e) f) Calculate to expected for this system, in minutes. (1) g) State Cmax for nitrate and Cas for sulfate in mM. Explain why these values are different. (2) Question 5=2+4+3+1+2+1+2-15 marks Sample NANG CHO Separator Suppressor H Celon-exchange mentrane KOH luent Anion separator column (strong- base anion exchanger in OH fon NOV Towe Anion chromatography Conductty Conductivity detector (K),50 Elion time I K'OH KO

Step by Step Solution

★★★★★

3.38 Rating (157 Votes )

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock

a The peaks in the top chrontegrum if Xo t h hi HH I t 0502 01 Chrom... View full answer

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Students Have Also Explored These Related Chemistry Questions!

Calculate the sodium ion concentration when 70.0 mL of 3.0 M sodium carbonate is added to 30.0 mL of 1.0 M sodium bicarbonate. Discuss.

A 25.0-mL sample of sodium sulfate solution was analyzed by adding an excess of barium chloride solution to produce barium sulfate crystals, which were filtered from the solution. Na2SO4(aq) +...

A solution containing calcium ion and magnesium ion is buffered with ammonia-ammonium chloride. When carbonate ion is added to the solution, calcium carbonate precipitates but magnesium carbonate...

Determine the force in members KJ, KC, and BC of the Howe truss, and state if the members are in tension orcompression. 6 kN 5 kN 4 kN 4 kN 5 kN 3 kN 4 m 2 kN G. JE IC D 2 m

Let A = {1, 2, 3} and B = {-2, - 1, 0, 1, 2, 3}. 64. The probability of increasing functions from A to B, is 1 1 5 7 27 54 (a) (a) 5 27 (b). 1/3 18 65. The probability of non-decreasing functions...

A small water pump is used in an irrigation system. The pump takes water in from a river at 10oC, 100 kPa at a rate of 5 kg/s. The exit line enters a pipe that goes up to an elevation 20 m above the...

Certain vegetables and fruits contain plant pigments called carotenoids that are metabolized in the body to produce Vitamin A. Lack of Vitamin A causes an estimated 250,000 to 500,000 children...

The cast-iron bell-crank lever depicted in the figure is acted upon by forces F1 of 250 lbf and F2 of 333 lbf. The section A-A at the central pivot has a curved inner surface with a radius of ri = 1...

Steady-state temperatures (K) at three nodal points of a long rectangular rod are as shown. The rod experiences a uniform volumetric generation rate of 5 X 107 W/m3 and has a thermal conductivity of...

Sodium bicarbonate is synthesized by reacting sodium carbonate with carbon dioxide and water at 70C and 2.0 atm gauge: Na 2 CO 3 + CO 2 + H 2 0 2 NaHCO 3 an aqueous solution containing 7.00 wt%...

Suppose a new standardized test is given to 97 randomly selected third-grade students in New Jersey. The sample average score Upper Y overbar Y on the test is 56 points and the sample standard...

Write a program to randomly generate integer data values into an array that can hold 25 values. The program should ask the user to enter the minimum and maximum values in a numeric range that is not...

5 . 5 5 What aspects of a corporate safety culture or a corporate ethics culture seemed to be missing at JCO? What would you do to improve this?

Consider the following data on 20 chemical reactions, with Y= chromatographic retention time (seconds) and X= molecular weight (gm/mole). Retention Time and Molecular Weight (n = 20) Retention...

Below are percentages for annual sales growth and net sales attributed to loyalty card usage at 74 Noodies & Company restaurants. Annual Sales Growth () and Loyalty Card Usage (* of Net Sales) (n =...

The power series 2" (171 )" An has radius of convergence 43. Which function does it converge to in its interval of convergence?

Alexis deposited $1,000 at the end of every month into an RRSP for 7 years. The interest rate earned was 3.50% compounded semi-annually for the first 3 years and changed to 3.75% compounded monthly...

4. Find cos dx. You can assume without proof that this integral converges. -1 Indicate the contour you use to evaluate this integral.

19. Which of the following techniques uses variables such as price and promotional expenditures, which are related to product demand, to predict demand? a. associative models b. Naive approach c....

For the separation of which of the following substances, Gas-solid chromatography is being used Select one: a. Low molecular weight gaseous species O b. Volatile organic components O c. Thermally...

On January 31, 2005, partners of Lon, Mac & Nan LLP had the following loan and capital account balances (after closing entries for January): The partnerships income-sharing ratio was Lon, 50%; Mac,...

please answer the question 7. What is the purpose of the suppressor column in lon Chromatography? (2 Marks)

10. Sea salt and pure water were prepared to make artificial seawater. We had two analysis tools. One was lon Chromatography (IC) instrument for [Cl-] analysis and the other was the conductivity...

If you intend to measure all the anions and cations in an unknown, one sanity check on your results is that the total positive charge should equal the total negative charge. The table lists...

Using the attached spreadsheet template as a model, you are going to first replicate the risk management strategy of using futures contracts to hedge aluminum prices. Next, create a risk management...