Question: question 1 & 2 pleassse it is based on gel filtration page 161-162 1. Why is it even more critical with gel filtration chromatography than

question 1 & 2 pleassse it is based on gel filtration

page 161-162 1. Why is it even more critical with gel filtration chromatography than the other chromatography methods you have used to have an absolutely level surface for the top of the gel bed in the column? 2. Why are we using just 1 ml of crude material instead of everything we have left? To answer this it may be helpful to think about the next question at the same time

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