Question 1 (20 points) Using the following data set, graph a Michaelis-Menten plot. Next, graph a Lineweaver-Burk plot and calculate both Km and Vmax (round to the nearest thousandth). Watch the video on how to make these graphs correctly in Excel. Use a scatter plot and show the best fit line for the Lineweaver-Burk plot. Make sure you have uploaded both values and graphs. [S] in mM Vo in uM/s 0 0 0.9 0.24 2.07 0.488 3.105 0.6 4.14 0.728 5.175 0.792 6.21 0.88 9.315 0.96 12.42 1 Question 2 (20 points) Using the following data set, graph a Michaelis-Menten plot. Graph a Lineweaver-Burk plot and calculate both Km and Vmax (round to the nearest thousandth). Next, make a Lineweaver-Burk plot with the enzyme and each inhibitor. Find the Km and Vmax for each inhibitor using the best fit equation. Based on the results, classify the type of inhibitor. Make sure to upload all three plots and the Km and Vmax calculations for 1) the enzyme 2) inhibitor 1 and 3) inhibitor 3. Inhibitor 1 Vo in Inhibitor 2 Vo in [S] in mM Vo in uM/s UM/s UM/s 0 0 0 0 0.25 0.5 0.2 0.43 0.5 0.875 0.35 0.795 0.75 1.25 0.5 1.14 1 1.375 0.55 1.265 1.25 1.475 0.59 1.365 1.5 1.575 0.63 1.445 1.75 1.6 0.64 1.47 2 1.625 0.65 1.495 Part 2 Upload a video explanation of your plots. Include an explanation of how you determined Km, Vmax and how you determined the type of inhibitor