The rate of movement of two elemental components E and F in a chromatographic column are...

 

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The rate of movement of two elemental components E and F in a chromatographic column are 1 x 10* mm³/s and 9 x 104cm/hr respectively. The distance covered by the mobile phase front is 1.0 m. The solute distribution ratio is 25 x 10-2 and the retardation factors are 3 x 10-4 and 5 x 10 2 for E and F, respectively and efficiency of the chromatography is 75%o. (i) Determine the volumetric flow rate of the solute concentration in the mobile phase. (ii) Calcurate (a) the time each component spent in the column (stationary phase) and mobile phase if the retention volume is 50 litres and the capacity factor is 0.05 x 10 (b) the distance travelled by each solute centre (c) the bandwidth of component E and F. The rate of movement of two elemental components E and F in a chromatographic column are 1 x 10* mm³/s and 9 x 104cm/hr respectively. The distance covered by the mobile phase front is 1.0 m. The solute distribution ratio is 25 x 10-2 and the retardation factors are 3 x 10-4 and 5 x 10 2 for E and F, respectively and efficiency of the chromatography is 75%o. (i) Determine the volumetric flow rate of the solute concentration in the mobile phase. (ii) Calcurate (a) the time each component spent in the column (stationary phase) and mobile phase if the retention volume is 50 litres and the capacity factor is 0.05 x 10 (b) the distance travelled by each solute centre (c) the bandwidth of component E and F.