Question $2$:

The diagram below shows the restriction map for a plasmid. Determine the sizes of the fragments when the plasmid is digested with HindIII and BamHI at the same time. $[2]$

Question #$3$:

A piece of DNA was cut using a restriction enzyme which produced fragments of the following sizes. $350bp,1300bp$ and $1800$ bp $.$

The fragments were then separated using gel electrophoresis. A molecular weight marker was loaded into lane $1$ and the digest fragments were loaded into lane $2.$

a$)$ The bands for the fragments of the molecular weight marker are shown in lane $1($fragment sizes are known to be $500bp,1000bp,1500bp,2000$ and $2500$ bp $).$ Label the fragment sizes for these bands in base pairs $($bp$)$ on the blanks beside the gel. $[1]$

b$)$ On the gel, sketch the bands that would be produced by the digest fragments. $[2]$

c$)$ The digest from question #$2$ were loaded into lane $3.$ Which bands would be visible $($draw them in$)?$ Which band$($s$)$ would not and why? $[3]$