QUESTION 2. You are studying a gene called Extreme ! in the bacterial species E coli that provides a slight advantage of better survival in high salt conditions. You have tested other species of bacteria in high salt conditions and observed that three other species are even more resistant to high salt than E. coll, You hypothesize that perhaps the increased advantage is due to altered regulation of the cereus A. faecalis C. freundii Extreme 1 gene or gene products in the three E. coli species. You collect samples from all four species and perform a Southern blot (top blot) -1500 bp e Northern blot (middle blot) and Western blot (bottom blot) and use the appropriate probes to visualize the entire Extreme 1 gene and ~750 bp gene products, Sizes of the various Extremet molecules are listed on the left of each blot -50 kDa 2a. (3 pts) What molecules are being analyzed in each of the three blots. Write the molecules next to the corresponding type of blot below. Southern blot = Northern blot = Western blot = 2b. (4 pts) Is your hypothesis correct for B. cereus? Circle one: Yes or No Explain your answer and be specific about what could cause any difference(s) you see between B. cereus and E. coli. 2c. (4 pts) Is your hypothesis correct for A. faecalis? Circle one: Yes or No Explain your answer and be specific about what could cause any difference(s) you see between A. faecalis and E. coli. 2d. (4 pts) Is your hypothesis correct for C. freundii? Circle one: Yes or No Explain your answer and be specific about what could cause any difference(s) you see between C. freundii and E. coli. 2e. (2 pts) There is a difference in the size of the molecule in the Southern blot versus the size of the molecule from the Northern blot. What could account for the difference