Question: Question 3 (20 marks) E. coli growth leading to cell mass concentration changes from 0.10g-dry cell/ L to 0.70g dry cell/L in 2 hours while

 Question 3 (20 marks) E. coli growth leading to cell mass

Question 3 (20 marks) E. coli growth leading to cell mass concentration changes from 0.10g-dry cell/ L to 0.70g dry cell/L in 2 hours while glucose is the main nutrient for cell growth. i) Assuming the exponential growth during this period of time, evaluate the specific growth rate: nct=g[5 points ] ii) Evaluate the doubling time during the exponential growth phase. [5 points] iii) How long would be required to grow from 0.80kg-dry cell /L to 1.30kg-dry cell/L? You may assume the exponential growth during this period. [5 points] iv) During the period of 2 hours as mentioned above, glucose concentration is reduced from 92g/L to 10g/L. Calculate the yield coefficient of cell growth. [5 points]

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