Question $3$

A technologist dissolved $57\mathrm{.}2mg$ of penicillin in ethanol to a volume of $132mL.$ From that solution, $1\mathrm{.}00mL$ was withdrawn using a

pipette and diluted to a $461mL$ with ethanol. Determine the concentration of penicillin in the final solution. Penicillin MW$=334\mathrm{.}4$

$\frac{g}{m}ol$

$A_1$

Question $4$

Acetate buffer $(pH=5\mathrm{.}3)$ is required for biopharmaceutical protein purification. Buffer has to be prepared by adding NaOH to a $1\mathrm{.}3L$

of $0\mathrm{.}88M$ aqueous acetic acid. Determine the weight of NaOH that needs to be added to acetic acid to make the required volume of

buffer. Acetic acid pKa$=4\mathrm{.}76$;$MW$ of NaOH$=40\mathrm{.}0\frac{g}{m}ol$