Question $51$

What sequence at the $3^{\text{'}}$ end of an extended guide RNA could be used to prime edit the sickle cell mutation?

HBB Sequence in Normal Adult Hemoglobin $($ HbA $)$:

Nucleotide $5^{\text{'}}$ CTG ACT CCT GAG GAG AAG TCT $3^{\text{'}}$

amino Acid

HBB Sequence in Mutant Adult Hemoglobin $($ Hb S $)$:

Nucleotide $5^{\text{'}}$ CTG$.$ ACT CCT GTG GAG AAG TCT $3^{\text{'}}$

O$)3$ CTGACTCCTGAGGAGAGTCTF

O$)$ GACUGAGGACJCUCUCUCAGA $5$

O$)$ GACTGAGGACTCCTCTTCAGA$5$

O $3$ CUGACUCCUGAGGAGAGGTCT$5$