Question: Question 5-7 5. Two samples of DNA have the same Tm and optical density (A260). Does this prove they are from the same species? Explain.

Question 5-7

Question 5-7 5. Two samples of DNA have the same Tm and

5. Two samples of DNA have the same Tm and optical density (A260). Does this prove they are from the same species? Explain. (2pts) 6. A sample of sea urchin DNA has 35 mole percent of G+C, and has a Tm of 86C. A sample of mold DNA has 50 mole percent of G+C and a Tm of 91C. Estimate the percent A+T content of a sample of unknown DNA with a Tm of 83C. (2pts) 7. You found that a diluted sample of a DNA plasmid had a concentration of 2.3ngmL1 after a 1/200 dilution. a. How much plasmid (in gmL1 ) was in the original solution? (lpt) b. What would be the A260 before 1/200 dilution? (lpt)

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