Question on Protein Thermal Stability (10 points) Let Tm be the temperature at the midpoint of a protein melting or thermal unfolding curve. Results from the analysis of thermal unfolding curves for a wild-type protein and two mutants are given below (AHm and Asm, are the enthalpy change and entropy change for the melting or thermal unfolding, respectively). Use this data to compute the Tm value (in K and then report them in Celsius) for each protein, and then to estimate the AAG (in kcal/mol) of the unfolding reaction for each mutant relative to the wild-type. Show your work if you wish to receive full credit Protein m ASm (kcal/mol) (cal/mol/deg) Wild-type 95 295 Mutant 1 110 335 Mutant 2 100 315