Question: questions 6 & 7 please 9) would be required. (3 marks) 6. TAE buffer is also used to run agarose gels. Approximately 270 mL of

questions 6 & 7 please

9) would be required. (3 marks) 6. TAE buffer is also used to run agarose gels. Approximately 270 mL of 1X TAE is required to run each gel (the volume required to reach the fill line on the gel running box). If you are a technician preparing to run 10 agarose gels, how would you prepare the total volume of 1X TAE needed (plus some excess in case of spills), using 50X TAE buffer? (2 marks) 7. You prepare a 7 x 10 cm gel with a volume of 35 mL of 1.0 % agarose. You get your gel ready to run, but when you load 20 ML of your sample into the well it overflows. Why did this happen? What might vou suggest changing to fix this

Step by Step Solution

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock blur-text-image

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Students Have Also Explored These Related Chemistry Questions!

5. If you were a technician who was required to prepare five agarose gels simultaneously, each with a final volume of 50 mL and an agarose percentage of 1.20%, determine what volume (in mL) of 10X...

You helped me a couple of weeks ago. I was wondering if i could setup a time for tomorrow that you could be available to help me with some questions. I will pay $20 per question. They are short and I...

CLOSING ARGUMENTS A Closing Argument should: 1. Repeat your theory and theme of the case 2. Summarize the evidence that preceded it 3. Relate the evidence to the law and the legal issues (i.e....

Opening Statement for Defense The 1948 U.S. Presidential election was a hard fight for Harry Truman. No one thought he had a shot and the folks at the Chicago Daily Tribune didn't think so either. At...

CLOSING ARGUMENTS A Closing Argument should: 1. Repeat your theory and theme of the case 2. Summarize the evidence that preceded it 3. Relate the evidence to the law and the legal issues (i.e....

Final Exam - HIMS 650 Fall 2015 SOLUTIONS Directions: Please answer all questions. There are 14 questions and the exam is worth 25% of your total grade. The questions are not equally weighted. The...

PROGRAMME HANDBOOK: JANUARY 2016 INTAKE ASSIGNMENT 2: HUMAN RESOURCES DEVELOPMENT Read the case study below and answer the questions that follow. National HRD in Finland, Russia, and South Africa...

Hi there, I need to answer question question three. I have completed part1 but haven't done part 2,3 and 4 for question 3: Financial statement analysis below i have attached my work which you will...

Need help with the document attached. The company that this paper is about is Nvidia. Project Descriptions SEC 10-K Paper You will be asked to select a company that is publically traded. You must...

Hello there Tutors, I was wondering if anyone can please read through the case study and answer question 1 and 2 at the back. That is what is the WACC and the divisional WACC for detergent. I really...

Write an SQL statement to create a database called "SportsClub". Enter answer here In the text field below, input the missing keyword (___) from the following SQL statement to create a table called...

You can afford $400 per month for a used motorcycle payment. You have found a bank that is willing to make a 6 year at 8.4% interest vehicle loan that compounds monthly. What is the most you can...

when an investor accounts for an investment in common stock

Please answer all. Grade: 1. Fill in blanks (20pts) (1) Convert 10110 (2) (binary) to decimal (2).Convert 101010110.01 (2) (binary) to hexadecimal (3). Minterm expression of F(X,Y,Z) = m(0,3,4,5),...

Know the flexibility of the written word and its power to convey an idea, and know how to make your words behave so that your readers will understand.

The board cannot act without a consensus of opinion.

Dont do what the village blacksmith did when he instructed his apprentice as follows: When I take the shoe out of the fire, Ill lay it on the anvil, and when I nod my head, you hit it with the...