Regarding the question I asked for assitance with bellow:

Part 1: Transcription and Translation:

1. Transcribe the following DNA sequence from HbA. 5' - AGT AAC GGC AGA CTT CTC CTC AGG AGT CAG GTG CAC CAT - 3'

mRNA:

5' AGU AAC GGC AGA CUU CUC CJC AGG AGU CAG GUG CAC CAU - 3'

2. Translate the following mRNA sequence. 5' - AUG GUG CAC CUG ACU CCU GAG GAG AAG UCU GCC GUU ACU - 3'

Amino acid protein:

Met-Val-His-Leu-Thr-Pro-Glu-Glu-Lys-Ser-Ala-Val-Thr

3. Transcribe the following DNA sequence from HbA. 5' - AGT AAC GGC AGA CTT CTC CAC AGG AGT CAG GTG CAC CAT - 3'

mRNA:

5' - AGU AAC GGC AGA CUU CUC CAC AGG AGU CAG GUG CAC CAU - 3'

4. Translate the following mRNA sequence. 5' - AUG GUG CAC CUG ACU CCU GUG GAG AAG UCU GCC GUU ACU - 3'

Amino acid protein:

Met-Val-His-Leu-Thr-Pro-Val-Glu-Lys-Ser-Ala-Val-Thr

Part 2: Genetic Mutations:

5. Given the following 5' to 3' RNA sequence, translate each codon into its respective amino acid working left to right in the 5' to 3' direction. Use the 3 letter amino acid.

AUG GAG GUC UUU AAG AGA CAU UUA GAU GUA GCC CUU AGU GAU GUU UAG

Amino acid protein:

Met-Glu-Val-Phe-Lys-Arg-His-Leu-Asp-Val-Ala-Leu-Ser-Asp-Val-Stop 6. One or more point mutations in this sequence. AUG GAG GUC UUU AAG AGA CAU UUA GAU GUA GCC CUU AGU GAU GUU UAG

Point mutation (Change the GAG to GUG:

AUG GUG GUC UUU AAG AGA CAU UUA GAU GUA GCC CUU AGU GAU GUU UAG Translate the mutated sequence:

Met-Val-Val-Phe-Lys-Arg-His-Leu-Asp-Val-Ala-Leu-Ser-Asp-Val-Stop 7. Now, a frameshift mutation by adding one or more bases. AUG GAG GUC UUU AAG AGA CAU UUA GAU GUA GCC CUU AGU GAU GUU UAG

Frameshift mutation (insert C after the first codon AUG):

AUG CGA GGU CUU UAA GAG ACA UUU AGA UGU AGC CCU UAG UGA UGU UUA G Translate the mutated sequence:

Met-Arg-Gly-Leu-Stop 8. A frameshift mutation by deleting one or more bases. AUG GAG GUC UUU AAG AGA CAU UUA GAU GUA GCC CUU AGU GAU GUU UAG

Frameshift mutation (delete the G codon from the second codon):

AUG AGG UCU UUA AGA GAC AUU UAG AUG UAG CCC UUA GUG AUG UUU AG Translate the mutated sequence:

Met-Arg-Ser-Leu-Arg-Asp-Ile-Stop

Explanation: Detailed explanation:

Part 1: Transcription and Translation

DNA to mRNA (Transcription): The DNA sequence is transcribed into mRNA. Notice that Thymine (T) in DNA is replaced by Uracil (U) in mRNA. This is the process where the genetic information in DNA is copied into a messenger RNA molecule.

mRNA: 5' AGU AAC GGC AGA CUU CUC CUC AGG AGU CAG GUG CAC CAU - 3' mRNA to Protein (Translation): The mRNA sequence is read in three-nucleotide units called codons. Each codon specifies a particular amino acid (or a stop signal). This process occurs on ribosomes, resulting in the synthesis of a polypeptide chain.

Amino acid protein: Met-Val-His-Leu-Thr-Pro-Glu-Glu-Lys-Ser-Ala-Val-Thr DNA to mRNA (Transcription): Another DNA sequence is transcribed. Again, T is replaced by U to create the mRNA molecule that carries the genetic code.

mRNA: 5' - AGU AAC GGC AGA CUU CUC CAC AGG AGU CAG GUG CAC CAU - 3' mRNA to Protein (Translation): This mRNA sequence is translated into an amino acid sequence. A single nucleotide difference in the mRNA (GUG instead of GAG) leads to a different amino acid (Valine instead of Glutamic acid) at that position in the protein.

Amino acid protein: Met-Val-His-Leu-Thr-Pro-Val-Glu-Lys-Ser-Ala-Val-Thr

Part 2: Genetic Mutations

Translation of RNA: The provided mRNA sequence is translated into its corresponding amino acid sequence, reading three bases (a codon) at a time from the 5' to 3' end. The sequence ends with a stop codon (UAG), which signals the termination of protein synthesis.

Amino acid protein: Met-Glu-Val-Phe-Lys-Arg-His-Leu-Asp-Val-Ala-Leu-Ser-Asp-Val-Stop Point Mutation: A single base change (G to U) within the GAG codon (coding for Glutamic acid) results in a GUG codon (coding for Valine). This type of mutation, where one nucleotide is substituted for another, can lead to a change in a single amino acid in the protein.

Mutated RNA: AUG GUG GUC UUU AAG AGA CAU UUA GAU GUA GCC CUU AGU GAU GUU UAG Translated mutated sequence: Met-Val-Val-Phe-Lys-Arg-His-Leu-Asp-Val-Ala-Leu-Ser-Asp-Val-Stop Frameshift Mutation (Insertion): The insertion of a single base (C) after the first codon (AUG) shifts the reading frame for all subsequent codons. This means that the ribosome will read a completely different sequence of three-base codons, leading to a drastically altered amino acid sequence downstream of the insertion. Often, frameshift mutations can introduce premature stop codons, resulting in a truncated (shorter) protein.

Mutated RNA: AUG CGA GGU CUU UAA GAG ACA UUU AGA UGU AGC CCU UAG UGA UGU UUA G Translated mutated sequence: Met-Arg-Gly-Leu-Stop Frameshift Mutation (Deletion): The deletion of one base (G) from the second codon (GAG) also causes a shift in the reading frame for all subsequent codons. Similar to an insertion, this leads to a completely different amino acid sequence downstream of the deletion and can also result in premature stop codons, producing a truncated protein.

Mutated RNA: AUG AGG UCU UUA AGA GAC AUU UAG AUG UAG CCC UUA GUG AUG UUU AG Translated mutated sequence: Met-Arg-Ser-Leu-Arg-Asp-Ile-Stop

Can I have assistance with these additional sequences and translating them for part 2?

1. Protein Sequence with Frame Shift Mutation (Addition)

2. RNA Coding Strand with Frame Shift Mutation (Deletion)

3. Protein Sequence with Frame Shift Mutation (Deletion)