Remember when Emilio read in the article that DNP was an $$uncoupler$$ of Ox Phos?$$What would that mean in terms of the ETC and ATP synthesis?

The structure of DNP$,$ has an aromatic ring that looks sort of like benzene $$ do you think it would be more soluble in water, or in a hydrophobic solvent?

DNP is a $$weak acid$$ due to its $$OH group. This means that some of the time it can shed its proton, becoming $$O$-+$ H$+.$ This is unlike HCl$,$ which is a strong acid, and thus completely dissociates in water. So$,$ think about the following scenario:

You have created some artificial membrane vesicles that have a higher pH inside than the aqueous solution outside. To container $1$ of these vesicles you add a little HCl$,$ and to the other $($container $2)$ you add some DNP and then measure the internal pH of the vesicles.

What do you predict would happen? $($Draw out your reasoning before answering the question$)$

The internal pH of the vesicles will:

Group of answer choices

decrease in both $1$ and $2.$

decrease in $1$ but remain unchanged in $2.$

remain unchanged in $1$ but decrease in $2.$

remain unchanged in both $1$ and $2.$