respond to this post and include reference such as website. One organism that can be detected via PCR is Human Papillomavirus (HPV). The sample is usually taken from cervical epithelial cells, collected during a Pap smear. To prepare for PCR, you'd first lyse the cells and purify the viral DNA, removing proteins and inhibitors. Next, you'd add primers specific to conserved HPV genes, DNA polymerase, nucleotides, buffer, and Mg. The reaction cycles (denaturation, priming, extension) amplify the viral DNA if present. The PCR product can then be visualized by gel electrophoresis to confirm infection. This method is widely used because PCR is highly sensitive and can detect very small amounts of viral DNA, allowing for early diagnosis and better patient outcomes