Question: Review the primers used for specificity and efficiency. It is crucial they target all potential antibiotic biosynthetic genes within your samples. You may want to

Review the primers used for specificity and efficiency. It is crucial they target all potential antibiotic biosynthetic genes within your samples. You may want to refer to existing literature or databases to align primers with known gene clusters in closely related species. Consider sequencing PCR products to confirm identity and ascertain whether you missed potential producers that were present in low abundance or due to other technical limitations

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