Question: Shown below is a binding isotherm for a protein and lead compound inhibitor. There is a tryptophan near the binding site, and the fluorescence from

Shown below is a binding isotherm for a protein and lead

Shown below is a binding isotherm for a protein and lead compound inhibitor. There is a tryptophan near the binding site, and the fluorescence from this residue increases upon binding the compound (shown on the y axis). A fixed concentration of protein (5 nM) was incubated with variable concentration of drug and fluorescence measurements were taken. At the highest concentration of inhibitor (2M), the fluorescence signal is 490 units (not shown on the graph). Estimate the value of KD and explain your reasoning

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