Question: Shown below is the restriction enzyme map (labels on the bottom) for the human YFG1 gene. Different regulatory sequences of the YFG1 gene are indicated
Shown below is the restriction enzyme map (labels on the bottom) for the human YFG1 gene. Different regulatory sequences of the YFG1 gene are indicated with arrows (labels on the top). Refer to this image when answering the following two questions. Promoter +1 ATG STOP .. .T HindIll YFG1 .... Kpvl Hindill Pstl EcoRI Hindill BamHI If you wanted to clone the complete open reading frame of the YFG1 gene into a cloning vector, based on the restriction enzyme map, which restriction enzyme(s) would be best to use for cloning the complete sequence of the open reading frame? Based on the restriction enzyme map and other information given in the image, which restriction enzyme cuts within a DNA sequence that is transcribed but not translated
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