Simple Direct and Negative Stain Lab Points to remember: All stained slides can be washed with soap and water in the sink. . Always use the BF (brightfield) setting on the microscope when you are examining a stained slide. With the exception of a few, all STAINED BACTERIA should be observed using immersion oil lens (100X). Simple Stain: Follow the Simple Staining procedure outlined in Ex.3 (5). Negative Stain: Follow the Negative -staining procedure outlined on the next page. DO NOT HEAT-FIX! Ex.3 (6) Compare your slide(s) to Fig. 3.88 on page 191 of the lab manual. Reagent used Reagent used Size of for Simple for Negative ONE cell Staining Staining Bacteria Morphology Arrangement at 1000X Sketch (1 x w) Bacteria Color Bacteria Color Use the Microns terminologies in Background Background chapter 4 of the Color Color textbook Staphylococcus epidermidis Bacillus megaterium Tavana, A. | BIOL 074