So, apparently the dilution was in order, .25- 8x, the .5- 4x, the third sample 2x and
Question:
So, apparently the dilution was in order, .25- 8x, the .5- 4x, the third sample 2x and the ,last sample sample no dilution? Also supposedly the vol. BSA was all 2 and i don't understand as to why nor the volume of the water. I am confused as to where to start and look for first because i keep on getting decimals that even when converted do not make sense. please help and explain if you can.
Prepare 4 samples with the indicated amount of BSA protein. Each sample will have a final volume of 20 μl, max loaded into the gel. You will need to add 4 μl of 5X sample buffer, therefore you will add 16 μl of BSA solution and/or water to bring the volume up to 20 μl. don’t pipette volumes less than 2 μl.
Financial Accounting an introduction to concepts, methods and uses
ISBN: 978-0324789003
13th Edition
Authors: Clyde P. Stickney, Roman L. Weil, Katherine Schipper, Jennifer Francis