solve

Identify the important things that must be done to prevent problems when performing protein purification using column chromatography. |_| Equilibrate the column with buffer and keep enough buffer in the column to avoid disrupting proper separation. [_] Dilute the sample with buffer to prevent the proteins from moving too quickly through the gel bed. (_] Load solutions slowly and carefully down the side of the column to avoid disrupting the gel bed. [_] Dye each protein a different color to avoid mixing up the location of the proteins in the gel bed