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Identify the important things that must be done to prevent problems when performing protein purification using column chromatography. Equilibrate the column with buffer and keep enough buffer in the column to avoid disrupting proper separation. Macmillan Learning Dilute the sample with buffer to prevent the proteins from moving too quickly through the gel bed. Load solutions slowly and carefully down the side of the column to avoid disrupting the gel bed. Dye each protein a different color to avoid mixing up the location of the proteins in the gel bed. Incorrect