solve this question by doing real analysis and add referances from real articles .

specify all the conditions numerically (temperature ,column type , height , injection volume etc. and send the solution in word document please .)and explain everything by giving reasons. its a design question so do a real numerical design based on real data and references .

IMPORTANT WARNING !!!!!please dont send me chatgpt answer without giving references..

show also how will we prepare samples in laboratory , from which components how much did we use and explain if we re in laboratory how will we do this experiment in real life? determine peak heieghts and concentration , explain really detailly which conditions are affecting hplc ? ( how to optimize them , colone type , detector type , injection volume , mobile phase type , composition , stationary phase , temperature , pH etc.)

6. a) Investigate what kind of column and under what conditions a mixture containing Glucose (A)+ Sucrose (B) + 1-Kestose (C) can be analyzed in HPLC. By searching the literature, determine the appropriate HPLC column and conditions which this mixture can be analyzed. b) Explain how the Sucrose (B) peak in this mixture can be determined.(qualitative analysis) c) Explain how the Sucrose ( B ) concentration in the mixture can be determined. (quantitative analysis)