Spectrophotometry Homework

A$)$ Plot the Standard Curve for the Maltose Determination Assay $($I$).$ Make sure to label your graph

appropriately and include $R^2$ and the equation for the best$-$fit line. According to your data, what is the molar

absorption coefficient for the product of the reaction $($glucose$)?$ Note: molecular weight of maltose is $342\mathrm{.}3$

$\frac{g}{m}ol.$

B$)$ Plot the Standard Curve for the Total Protein Determination Assay $($II$).$ Make sure to label your graph

appropriately and include $R^2$ and the equation for the best$-$fit line. Does the data agree with the Beer$-$Lambert

law? Explain your response.

C$)$Plot the Absorption Spectrum for the data provided for $KMn{O}_4.$ What does the reading at $525nm$

represent? If the molar absorption coefficient for $KMn{O}_4$ is $174\mathrm{.}4M^{-1}c{m}^{-1},$ what was the concentration of the

stock solution?