Standard Phosphate Solution

Wavelength: Absorbance:

375 --------- 1.497 (value for step 6: Ar)

385 ---------- 1.101

395 ---------- 0.712

405 ----------0.602

415 --------- 0.518

Ammonium Vanadomolybdate + DI water

Wavelength: Absorbance:

375 ------------ 0.182 (value for step 5)

Fertilizer at 375 [Wavelength] Absorbance

1.10 g/L -------------------------- 0.531

2.37 g/L -------------------------- 0.798

2.270 g/L ------------------------- 1.125

In the Calculations section show the calculation of (l) from the absorption and concentration f the standard solution. For the fertilizer sample, show the calculation of the concentration of the iluted solution from the absorbance and (l) values, the conversion of this concentration to the riginal concentration by taking into consideration the dilution, and the calculation of the %P2O5. or one of the energy drink samples, show the calculation of the concentration of the diluted olution from the absorbance and (l) values, the conversion of this concentration to the original ottle. The following outlines the necessary calculations: Subtract the AR value (step 6 of the calibration procedure) from the absorbance of the standard solution at the wavelength used for the rest of the measurements. (The absorption data for the standard was collected in step 5 of the calibration procedure.) This gives AP for the standard solution. Rearrange the Beer-Lambert equation to solve for ( E l). Plug in the above AP value and the given concentration of the standard solution to calculate (l). You will use this value of (l) to calculate the concentration of phosphate in the fertilizer Subtract the absorbance of the reagent (AR) from the total absorbance (AT) for each reading of the fertilizer to calculate AP for each reading: AP=ATAR Rearrange the Beer-Lambert equation to solve for concentration (C). Solve for the concentration of phosphate in each fertilizer by plugging in the calculated (1) value and the corrected absorbance value (AP) for the sample