Student B did not have issues running column chromatography like Student A. Student B moved on and developed their TLC plate, marked the solvent front, and calculated Rf values for the spots. The table below shows the Rf values for samples found in each lane for Student B What explanation can you pose for the sample at Rf = 0.09 which was present in the extract sample but was not seen in the yellow or green band? If it was not collected in Fraction 2 or 4, where would it have ended up? Extract Lane Yellow Lane Green Lane Rf = 0.85 Rf = 0.85 -- Rf = 0.45 -- Rf = 0.45 Rf = 0.30 -- Rf = 0.30 Rf = 0.09