STUDYING CELLULAR RESPONSES TO HEAT SHOCK:

While studying the way cells react to heat shock $($exposure to $43$@ for four hours$),$ you received antibodies against $7$

different celulur proteins and some information related to the sequence of the proteins they recognize. The protein

information is under "Protein sequence data" and provides the length of the protein $($in number of amino acid residues$),$

the presence of specific amino acid sequences that could mediate targeting to specific cellular lo

and the presence of cleavabile signals.

Using the seven antibodies at hand, you performed

immunofluoresconce analyses of cultured A$549$ cells determine the location of the protoiturs both under normal cell

culture conditions $(37C),$ as well as after $4$ hours at $43C.$ However, you outained good immunofforerscecnce data only

for $3$ protetins $($identified as Protein $1,$ Protein $2,$ and Protein $3$ in the Immunofiuorescence data set to the right$).$ You also performed immunoblot analyses using cell lysates

dierived from $A$ A$549$ cells grown at normal conditions $(0$ h at $43C),$ as well as cells grown at $43C$ for $2$ hours and $4$

hours. You obtained good immunoblot data for all seven proteins $($see Immunoblot data below$).$ Whis point, answer the questions given in the all you know at Keep in mind that you will get $10$ questions, each worth $2$

points. You will get two chances at answering the you can choose to answer it only once. The hinhest radade

will be the one used toward your extra credit that will be added to your final grade.

$q,$ presented in the "Protein sequence data" with all the

proteins shown on the "Immunoblot data", and you should,

also try to match Protein $1,$ Protein $2,$ and Protein $3$ to both, the "Protein sequence data" and the "Immunoblot data"

Immunofhwrescence dato:

Protein sequence data:

Immunoblot data: $($ h@ $43C$ represents cells yrown @ $37C)$

$1^{2}34567891011121314151617181920212223$

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a Question Completion Status:

$10$

$20$

$30$

$40$

$5$

$6$

$70$

$8$

$90$

$10$ I

Moving to another question will save this response.

Question $1$

The protein that corresponds to the immunoblot data labeled as $16$ is

X$3$

X$6$

X$2$

X$5$

$x7$

$x4$

X$4$

X $4$

X $1$

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Question Completion Status:

$10$

$24$

$36$

$40$

$50$

$60$

$70$

$80$

$90$

$100$

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Question $2$

The protein$($s$)$ that does not $($do not$)$ appear to be affected at all by heat shock according to ALL the data provic

$x4$ and $x7$

$X7$

$x_3$

$x6$

$x2X_4$andX$7$

$x4$

$x2$

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Question Completion Status:

$10$

$29$

$(5)$

$49$

$50$

$69$

$70$

$80$

$90$

$100$

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Question $3$

The protein that corresponds to the immunoblot data labeled as $12$ is

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Question Completion Status:

$10$

$25$

$34$

$40$

$50$ Nm

$6$

$70$

$80$

$90$

$100$

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Question $5$

The protein$($s$)$ that appear$($s$)$ to be post$-$translationally modified by N $-$glycosylation is $($are$)$

X$3$ and X$4$

$"$X$1,$ X$3,$ and X$4"$

$"$X$1,$ X$2,$ X$3,$ and X$4"$

$x1$ and $x_4$

X $1$ and X $3$

X$3$

$x_1$

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Question Completion Status:

$19$

$20$

$34$

$45$

$50$

$6$

$70$

$85$

$95$

$100$

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Question $6$

This represents a pair of proteins that are very unlikely to interact with each other

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Question Completion Status:

$$

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Question $7$

The protein that corresponds to PROTEIN $1$ in the immunofluorescence data presented is

$$ X$4$

X$2$

X$7$

X$6$

X$1$

X$3$

X$5$

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