Question: Substrate Concentration (ug/mL) vs Initial Velocity (AA450/60 seconds) 50 100 150 200 250 300 Substrate Concentration (ug/mL) I what does it mean? Does it agree

 Substrate Concentration (ug/mL) vs Initial Velocity (AA450/60 seconds) 50 100 150

Substrate Concentration (ug/mL) vs Initial Velocity (AA450/60 seconds) 50 100 150 200 250 300 Substrate Concentration (ug/mL) I what does it mean? Does it agree with what we know about enzyme kinetics and why or why not? Comment on if the enzyme was indeed saturated or not? Why/why not and how can we tell from the obtained data? Was an optimal substrate concentration identifiable? Why or why not? Initial Velocity (AA450/60 seconds) 0.0018 0.0016 0.0014 0.0012 0,001 0.0008 100006 0.0004 0.0002 0 0 : 350 400

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