Question: summerize into lab steps A specimen was obtained from the unknown sample. The sample bacteria tested as Gram-negative bacillus through a Gram staining procedure. Inoculation

summerize into lab steps

A specimen was obtained from the unknown sample. The sample bacteria tested as Gram-negative bacillus through a Gram staining procedure. Inoculation of Agar Plates: Before proceeding with the work area and wire loop, the Bunsen burner flame was used to sterilize them. The laboratory technician used a wire loop to acquire a tube sample before making multiple plates on MacConkey agar. The wire loop received secondary sterilization before 1 performed a streak procedure on the blood agar plate. I placed the wire loop under re-sterilization before switching off the Bunsen burner. I incubated the agar plates at 37 degrees Celsius under 5% CO2 gas conditions. I used MacConkey agar for lactose fermentation and blood agar for hemolysis results. The laboratory disposition included proper plate discarding into the waste bin. Performing Biochemical Tests (Oxidase, Indole, and Catalase): Catalase Test:

6 Copyright Labster ApS 2021 All Rights ReservedI sterilized the workspace by activating the Bunsen burner. The Bunsen burner was used to sterilize the wire loop. Added hydrogen peroxide into the test tube. A bacteria sample was obtained using the sterilized wire loop before being placed into the hydrogen peroxide. The test tube observation of bubble formation showed whether the bacteria contained catalase. A discarded test tube following sterilization of the wire loop. Oxidase Test: Test strips for the oxidase test were placed adjacent to the Bunsen burner. A wire loop was first autoclaved, and then laboratory bacteria were obtained by contacting the loop with the test strip. A sterilized wire loop was used to observe color changes on the strip and identify oxidase test results. I disposed of the test strip while also switching off the Bunsen burner. Indole Test: A bacteria sample was obtained using a sterilized wire loop to transfer material onto the indole test strip. After sterilization, I checked the test strip for color changes using a wire loop. The test strip was disposed of shortly after the burner received a power shutdown

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