Question: Table 6-8: Purification Table for GST-GFP 1 2 3 4 5 6 7 8 9 Protein Total Total Reaction Total Specific Percent Percent Fold Conc.

Table 6-8: Purification Table for GST-GFP 1 2 3 4 5 6 7 8 9 Protein Total Total Reaction Total Specific Percent Percent Fold Conc. Volume Protein Velocity Activity Activity Yield Yield Purif. (mg/ml) (ml) (mg) (units/ml) (units) (units/mg) (Total) (GST-GFP) (GST-GFP) A Lysate 0.3 6.5 1.95 0.148 0.962 3.20 100 100 Affinity B 0.6 3.0 1.80 Elution 0.113 0.339 0.565 35.2 0.176 IEX C 1.2 1.0 1.2 0.053 Elution 0.053 0.044 5.51 0.014

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