TAKE SHORT CONCISE IMPORTANT NOTES ON [PASCO Spectrometer: Kinetics] 0:08 Hi. This is Tom Loschiavo, Chemistry Education Manager at PASCO scientific. 0:12 I'm here today to talk about how you can do some kinetics experiments 0:15 with the PASCO Spectrometer. 0:19 I have the PASCO Spectrometer Bluetooth-connected to my computer. 0:22 I have the Spectrometry software open and ready to go. 0:26 Everything is paired correctly. 0:28 Today, I'm going to do a brief experiment 0:31 using some crystal violet and some sodium hydroxide. Calibration 0:35 In order to do this, we're going to need to calibrate the spectrometer. 0:41 We're going to go to the Analyze Solution page. 0:46 I'm going to take a blank cuvette of my solvent, in this case water. 0:51 I'm going to put it in the spectrometer 0:52 so the clear side of the cuvette is facing the detector and the light. 0:56 It's going to fit in there snugly, 0:57 so it doesn't wiggle around while you're doing your experiment. 1:01 I'm going to calibrate a dark reference, 1:04 which means the light will be turned off in the spectrometer. 1:09 I'll calibrate a light reference, where the light will be on, 1:12 going through the solvent to effectively blank out the solvent. 1:18 After it's done calibrating, I'm going to hit Start 1:22 to make sure it calibrated correctly. 1:24 I have a nice flat line at zero absorbance for my solvent, 1:27 which is what I want. 1:30 For my crystal violet, I have a sample of crystal violet in a cuvette. 1:34 I