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8:22 all 5G+ Done api.playposit.com 2 C How do we detect methylated sites? 1. Gene sequencing original DNA - only bases |Bisulfite conversion read (no idea where methylation occurs) Che | sequenceh 3 C GATI ACG TT Bisulfite-treated 2. Bathe DNA with sequence CGATCACG TC Reference genome (untreated) sodium bisulfite = transforms unmethylated These Cs were methylated to U C GATCAC ETC 3. Compare original CC The following DNA segment has been sequenced with and without sodium bisulfate. What would the sequence including methylation patterns be? (C* represents a methylated cytosine) Sequence without sodium bisulfate: CCGCTCAAGCTAC Sequence with sodium bisulfate: CCGTTTAAGTTAC Sequence including methylation patterns: CC*GCTCAAGCTAC 0 / 3 pts RETRY CONTINUE 15:44 1:09:28