The avian gene fluegel-5 (f5) encodes a transcription factor and is expressed in developing forelimbs (but not
Question:
The avian gene fluegel-5 (f5) encodes a transcription factor and is expressed in developing forelimbs (but not hindlimbs) during chick development. This and other evidence suggests that the F5 protein might regulate gene networks involved in the generation of wing structures.
Question 1. Formulate a hypothesis regarding the possible function of F5 in directing limb identity
1(a). Describe an experiment to determine if F5 is necessary for forelimb development
1(b). Describe a result that is consistent with the hypothesis that F5 regulates the expression of genes that produce the avian forelimb.
1(c). Describe a result that is inconsistent with this hypothesis.
1(d). Describe an experiment to determine if F5 is sufficient to cause wing development.
1(e). Describe a result that is consistent with sufficiency.
1(f). Describe a result that is inconsistent with sufficiency