The book describes the polymerase chain reaction. A) If one of the primers has a point...

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The book describes the polymerase chain reaction. A) If one of the primers has a point mutation distinct from the template what is the percentage of double- stranded DNA that contains a single point mutation after 3 PCR cycles (melt-anneal-polymerize)? B) Illustrate where you would place primers, and in what orientation, to amplify circular double-stranded DNA (arrows along strands to indicate 5' 3' direction)? Note this necessarily creates linear DNA. Is the DNA amplification linear or exponential? The book describes the polymerase chain reaction. A) If one of the primers has a point mutation distinct from the template what is the percentage of double- stranded DNA that contains a single point mutation after 3 PCR cycles (melt-anneal-polymerize)? B) Illustrate where you would place primers, and in what orientation, to amplify circular double-stranded DNA (arrows along strands to indicate 5' 3' direction)? Note this necessarily creates linear DNA. Is the DNA amplification linear or exponential?

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Mutation Mutation are generally referred to as replacement of another base pair in particular positi... View the full answer