2. (a) The concentration of competent cells added to each of the tubes was 10⁹ cells/ml. Calculate

the percentage of ALL cells taking up DNA for the transformation in Tube A at step 4.

2(b) The concentration of competent cells added to each of the tubes was 10⁹ cells/ml. Calculate the percentage of transformed cells (which contain the catechol – 2 3 dioxygenase gene) in Tube A. at step 4

Relevant Class data

Average number of transformants in class A Neat	Average number of transformants in classA 10 -1	Average number of colonies in class A neat Y	Average number of colonies in classA 10-1 Y	Average number of transformants in classB Neat	Average number of transformants in classB 10-1	Average number of colonies in classB Neat Y	Average number of colonies in classB 10-1 Y	Average number of transformants in classC Neat	Average number of transformants in classC 10-1	Average number of colonies in classC Neat Y	Average number of colonies in classC 10-1 Y
320	75	19	2	224	0	9	0	53	14	4	2
816	160	53	3	520	47	7	1	200	12	2	0
292	34	11	0	171	33	1	1	129	7	3	0
394	110	12	3	204	59	5	1	55	15	3	0
115	24	2	0	78	23	4	1	42	60	0	0
448	59	15	1	420	36	7	2	308	49	6	4

Answer rating: 100% (QA)

Class A Avarage colonies found 3 Transformation effic... View the full answer