Question: The following data was shared by a previous colleague from a batch experiment where an optimised E . coli strain is producing antibiotics. The amount
The following data was shared by a previous colleague from a batch experiment where an optimised coli strain is producing antibiotics. The amount of substrate provided was reactor.
tableTime mincells
a Determine the following:
Specific growth rate
Doubling time
Length of the lag phase
b You conduct followup experiments with different substrate concentrations and obtain the following data.
tableTime min
What is the Michaelis Menten constant for this strain?
c You want to achieve a specific growth rate of to optimise the antibiotics production in a continuous culture reactor chemostat What should your flow rate into the chemostat and concentration of substrate in the feed be to achieve this?
d You've discovered the following data from a previous experiment in trying to optimise the substrate feed for antibiotics production:
Specific substrate consumption rate
Maintenance requirements
Yield coefficient of biomass vs substrate
Experimental data: product vs substrate yield
tableSubstrate fed Product produced
What would be the specific rate of product formation for the chemostat reactor in the previous question?
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