The following images represent confocal images of cells with full-length Mira protein (Mira 1- 830), which...

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The following images represent confocal images of cells with full-length Mira protein (Mira 1- 830), which localizes to the plasma membrane, and truncations of the Mira protein that either localize to the plasma membrane and/or the nucleus. How would you determine whether the membrane localization sequence of Mira is sufficient? HA-Mira ο οοο ο Mira 1-830 Mira 1-727 B A Mira 1-648 Mira 1-405 Mira 1-329 Mira 1-290 D Add amino acids 94-290 from Mira to GFP and examine localization of GFP Remove amino acids 94-290 from Mira and examine the localization of the protein Remove amino acids 1-106 from Mira and examine the localization of the protein Add amino acids 1-830 from Mira to GFP and examine localization of GFP Add amino acids 1-106 from Mira to GFP and examine localization of GFP Mira 1-106 Mira 94-290 H G The following images represent confocal images of cells with full-length Mira protein (Mira 1- 830), which localizes to the plasma membrane, and truncations of the Mira protein that either localize to the plasma membrane and/or the nucleus. How would you determine whether the membrane localization sequence of Mira is sufficient? HA-Mira ο οοο ο Mira 1-830 Mira 1-727 B A Mira 1-648 Mira 1-405 Mira 1-329 Mira 1-290 D Add amino acids 94-290 from Mira to GFP and examine localization of GFP Remove amino acids 94-290 from Mira and examine the localization of the protein Remove amino acids 1-106 from Mira and examine the localization of the protein Add amino acids 1-830 from Mira to GFP and examine localization of GFP Add amino acids 1-106 from Mira to GFP and examine localization of GFP Mira 1-106 Mira 94-290 H G

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