The forward and reverse primers for PCR amplification have thefollowing sequences: Forward: 5'-CTATGAGTTACTCACATG-3', Reverse:5'- GAAAGCATCCAGGCCCGG-3'. Based on

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Question:

The forward and reverse primers for PCR amplification have thefollowing sequences: Forward: 5'-CTATGAGTTACTCACATG-3', Reverse:5'- GAAAGCATCCAGGCCCGG-3'. Based on the manually calculated meltingtemperatures, would you recommend using these primers together? Whyor why not?