An unknown bacterium is tested for tryptophanase activity. The media used to test the organism was...
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An unknown bacterium is tested for tryptophanase activity. The media used to test the organism was prepared according to the following recipe: To 1000 mL distilled water add: • 5 g Lysine • 1 g Glucose • 5 g Acid Hydrolyzed Peptone • 3 g Yeast Extract • 0.02 g Brom Cresol Purple The inoculated media is incubated for 48 hours at 37°C. Following incubation, the media is briefly mixed by vortexing and Kovac Reagent added. The resulting assay for tryptophanase activity is shown below: Intepret the results: O The bacterium is negative for tryptophanase activity. The bacterium is positive for tryptophanase activity. O The result cannot be determined. O the bacterium did not grow in the media. Submit Answer Tries 0/99 An unknown bacterium is tested for tryptophanase activity. The media used to test the organism was prepared according to the following recipe: To 1000 mL distilled water add: • 5 g Lysine • 1 g Glucose • 5 g Acid Hydrolyzed Peptone • 3 g Yeast Extract • 0.02 g Brom Cresol Purple The inoculated media is incubated for 48 hours at 37°C. Following incubation, the media is briefly mixed by vortexing and Kovac Reagent added. The resulting assay for tryptophanase activity is shown below: Intepret the results: O The bacterium is negative for tryptophanase activity. The bacterium is positive for tryptophanase activity. O The result cannot be determined. O the bacterium did not grow in the media. Submit Answer Tries 0/99
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Related Book For
Chemistry The Central Science
ISBN: 978-0321696724
12th edition
Authors: Theodore Brown, Eugene LeMay, Bruce Bursten, Catherine Murphy, Patrick Woodward
Posted Date:
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