The Michaelis-Menten equation models the hyperbolic relationship between [S] and the initial reaction rate V, for an enzyme-catalyzed, single-substrate reaction E +S = ES E+P. The model can be more readily understood when comparing three conditions: [S] > Km: Match each statement with the condition that it describes. Note that "rate" refers to initial velocity V, where steady state conditions are assumed. [Etotal] refers to the total enzyme concentration and [Efree) refers to the concentration of free enzyme. [S] > Km Not true for any of these conditions Answer Bank Half of the active sites are filled with S. Adding more S will not increase the rate. Increasing [Exotal] will lower K... Almost all active sites are empty. [ES] is much higher than [Efr]. The rate is directly proportional to [S]